Mass spectrometry is an analytical method that uses the determination of the mass to charge ratio of the tested sample ions for analysis. The analyzed sample first needs to be ionized, and then different ions are separated by mass to charge ratio (m/z) based on their different motion behaviors in electric or magnetic fields to obtain mass spectra. Through the mass spectra and related information of the sample, qualitative and quantitative results can be obtained.
Chromatography, also known as chromatography or chromatography, is a physical and chemical analysis method that utilizes the differences in forces (distribution, adsorption, ion exchange, etc.) between different solutes (samples) and the stationary and mobile phases. When the two phases move relative to each other, each solute undergoes multiple equilibria between the two phases, achieving mutual separation. Its English name is chromatography, which comes from the Greek words chroma and graphein. When translated into English, it means color and writing, and when translated into Chinese, it means chromatography. But some people also translate it as chromatography or color chromatography.
Spectrum is a pattern of monochromatic light dispersed by a dispersive system (such as a prism or grating) and arranged in sequence according to wavelength (or frequency). Light waves are generated by electrons moving inside atoms, and the motion of electrons inside atoms varies among different substances, resulting in different emitted light waves.
The four major spectra are:
Nuclear magnetic resonance (NMR), mass spectrometry (MS) of material particles, vibrational spectroscopy infrared/Raman (IR/Raman), electronic transition ultraviolet (UV).
UV: Four absorption bands, generation, wavelength range, absorption coefficient
Infrared: characteristic peaks, factors affecting absorption peaks, and the relationship and differences between different compound spectra
NMR: N+1 rate, chemical shift influencing factors, chemical shift of various compounds
Mass spectrometry: characteristic ions, rearrangements, mass spectrometry characteristics of various compounds (such as the presence or absence of molecular ion peaks, etc.)
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